Bioavailability of zinc sources in chickens determined via real time polymerase chain reaction (RT-PCR) assay for metallothionein
نویسندگان
چکیده
Tissue zinc is used to define zinc availability, but deposition is far from the absorption site and only a fraction of absorbed zinc is deposited. Alternatively, zinc availability may be defined by quantifying expression of specific zinc-responsive genes in the small intestine. Metallothionein (MT) protein binds zinc and its mRNA expression is reported to reflect zinc status. A real time polymerase chain reaction (RT-PCR) assay was developed to measure MT mRNA expression in chickens fed titrated levels or sources of zinc. MT was inducible by zinc in small intestine, liver and kidney. Broilers were fed a milo-soy diet for 20 days, then were provided an unsupplemented corn-soy basal diet or the basal with 70ppm zinc from zinc oxide (ZO), a zinc amino acid complex (ZAAC) or MINTREX® Zn. Jejunum scrapings were collected after two days and assayed for MT expression. ZO and ZAAC failed to induce MT expression significantly above the basal. Birds fed MINTREX Zn exhibited MT expression significantly greater than all other treatments, suggesting that MINTREX Zn is more bioavailable than ZO or ZAAC. Subsequent commercial trials with reduced (50%) mineral inputs via MINTREX support equal live performance.
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